Role of Mucus Secretion and Sulfhydryl Groups in Gastroprotection Mediated by a Flavonoid Fraction of Bidens aurea

C. La Casa, M. J. Martin Calero, C. Alarcon de la Lastra, V. Motilva, M. J. Ayuso, C. Martin Cordero and A. Lopez Vergara Departamento de Farmacia y Tecnologfa Farmaceutica. Laboratorio de Farmacologfa, Faeultad de Farmacia, C/Prof. Garcia Gonzalez s/n. 41012-Sevilla. Spain Z. Naturforsch. 50c, 854-861 (1995); received June 1/August 15, 1995 Cytoprotection, Bidens aurea , Sulfhydryl Groups, Aurones, Chalcones The aerial parts of Bidens aurea (Aiton) Sherff have been used in folk medicine as an antiulcer agent. The efficacy of an ether extract to prevent gastric mucosal damage produced by several necrotic agents was studied in rats. The best efficiency was against lesions induced by 30% NaCl with a significant decrease of damage at all doses tested (250, 125 and 62.5 mg kg '1). In contrast, only the highest dose (250 mg kg"1) was effective on gastric erosions in­ duced by 0.6 n HC1. This effect could not be related with the mucus gel production only since the groups treated with of absolute ethanol or 30% NaCl before and then received 250 mg kg"1 of the ether extract increased significantly the amount of gastric mucus and its glycoprotein content. However, the ether extract of Bidens aurea enhanced the production of mucosal non-protein SH groups at all doses assayed after treatment with absolute ethanol and 0.6 n HC1. With NaCl-induced gastric injury, also the highest dose (250 mg k g 1) pro­ duced an increase of this parameter. The results suggest that a slight augmentation of the mucus gel concentration and the increase of non-protein sulfhydryl compounds, could con­ tribute to the functional protection mechanism mediated by a flavonoid fraction of B.aurea in these experimental models.